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2015 Fiscal Year Research-status Report

Phenotypic and genotypic disparity in Sri Lankan primates: Dissecting a species from its morphology, ecology, parasites and genes

Research Project

Project/Area Number 26440258
Research InstitutionKyoto University

Principal Investigator

M・A Huffman  京都大学, 霊長類研究所, 准教授 (10335242)

Co-Investigator(Kenkyū-buntansha) 川本 芳  京都大学, 霊長類研究所, 准教授 (00177750)
Project Period (FY) 2014-04-01 – 2017-03-31
Keywordsphylogeography / speciation / phenotypic traits / genotypic variation / toque macaque / purple face langur / grey langur / Sri Lanka
Outline of Annual Research Achievements

In H27 we were successful in accomplishing all research goals. Based on our continual updating and evaluation of results the three following major results were obtained: 1) Inter-population variation in tail length in toque macaques (TM) was noticed, length decreasing with altitude. To assess this trait and its possible sub-species variation, we developed a non-invasive method for assessment of this phenotypical trait using full-body-profile photographs of monkeys to quantify the relative lengths of tail and trunk across populations using digital images. Quantification is done using 'pixel distance' derived from mouse drawn lines along the contour of tail and trunk. This method allows us to calculate a ratio of two morphological traits, without the need to control for distance from the monkey. Validation of this method is underway based on 172 TM photos taken in the H26 and H27 field seasons from 23 different troops representative of the three phenotypically classified TM sub-species and secondarily grouped into our two recognized haplotype clusters A & B. 2) Primers have been made and tested to sample for macaque malaria parasites. These will be used to investigate possible TM sub-species - malaria co-evolution. 3) Several populations of PFL and GL color morph have been discovered and fecal samples are being collected to investigate the possibility of regional specific candidate genes (MC1R) for coat color mutation. So far, there is no report on the coat color mutant caused by this gene in non-human primates. It may provide new perspectives on sub-species differences.

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

The establishment of a methodology for non-invasive morphometric measurement to investigate possible regional, altitudinal and sub-species differences in phenotypical traits, the development of primers for genetic classification of macaque malaria and the discovery of color morphs among sympatric PFL and GL will now allow us to evaluate the phenotypic and genotypic disparity of Sri Lankan primates. A preliminary comparative DNA study was conducted for the PFL and GL. There was inconsistency between morphology and mtDNA types. In the cluster analysis, some of PFL formed a cohesive inner cluster but GL showed a mixed inner cluster with PFL types. Additionally, there were some very divergent mtDNA types forming outer clusters. This finding of inconsistency is a new important research subject which be useful in elucidating the evolutionary background of non-human primates in Sri Lanka.

Strategy for Future Research Activity

Since last year, in collaboration with our local counterpart in Sri Lanka, long-term monitoring at Mihintale, a study site where the TM, PFL and GL live sympatrically, has begun and systematic sampling of behavior, ecology, and intestinal parasites have begun. This will enhance our understanding of inter-species interactions at contact zones. Our island wide survey will also continue. Phylogeographical characteristics of langurs will be investigated by increasing the survey sites to evaluate biological boundaries or areas of mixing of focal morphological and molecular traits. Finally, zoogeographical features of Sri Lankan non-human primates will be synthetically compared with the research results of our colleagues studying macaques and langurs in other South and Southeast Asian countries in the fall. Between October 17~19, 2017 we will co-host an international symposium where the results of our research program will be presented.

  • Research Products

    (10 results)

All 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Int'l Joint Research] University of Sri Jayawardenepura(Sri Lanka)

    • Country Name
      Sri Lanka
    • Counterpart Institution
      University of Sri Jayawardenepura
  • [Int'l Joint Research] National Institute for Advanced Studies(India)

    • Country Name
      India
    • Counterpart Institution
      National Institute for Advanced Studies
  • [Int'l Joint Research] Tribhuvan University(Nepal)

    • Country Name
      Nepal
    • Counterpart Institution
      Tribhuvan University
  • [Journal Article] An SNP marker at the STAT6 locus can identify the hybrids between rhesus (Macaca mulatta) and long-tailed macaques (M. fascicularis) in Thailand: a rapid and simple screening method and its application.2016

    • Author(s)
      Jadejaroen J, Kawamoto Y, Hamada Y, Malaivijitnond S
    • Journal Title

      Primates

      Volume: 57 Pages: 93-102

    • DOI

      10.1007/s10329-015-0502-2

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular identification of Oesophagostomum spp. from ‘village’ chimpanzees in Uganda and their phylogenetic relationship with those of other primates.2015

    • Author(s)
      Ota N, Hasegawa H, McLennan MR, Kooriyama T, Sato H, Pebsworth PA, Huffman MA.
    • Journal Title

      Royal Society Open Science

      Volume: 2: 150471 Pages: 1 - 12

    • DOI

      http://dx.doi.org/10.1098/rsos.150471

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Maxillary sinus variation in hybrid macaques: implications for the genetic basis of craniofacial pneumatization.2015

    • Author(s)
      Ito T, Kawamoto Y, Hamada Y, Nishimura T
    • Journal Title

      Biological Journal of the Linnean Society

      Volume: 115 Pages: 333-347

    • DOI

      10.1111/bij.12528

    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Genetic structure of island populations of crab-eating macaques in Indonesia and Japanese macaques2015

    • Author(s)
      Kawamoto Y
    • Organizer
      International Seminar: “Nonhuman Primates in Biology, Conservation and Biomedical Research” in Indonesia
    • Place of Presentation
      Bogor, Indonesia
    • Year and Date
      2015-08-31 – 2015-08-31
  • [Presentation] The Evolution of Self-Medication in Primates from a Cross Taxa Comparative Perspective2015

    • Author(s)
      M.A. Huffman
    • Organizer
      European Federation for Primatology
    • Place of Presentation
      Science Department, Roma Tre University, Rome Italy
    • Year and Date
      2015-08-25 – 2015-08-28
    • Int'l Joint Research / Invited
  • [Presentation] The ‘grey zone’ of langur phylogeography in Sri Lanka2015

    • Author(s)
      M.A. Huffman, Y. Kawamoto, C.A.D. Nahallage, R. Kumara, T. Shotake
    • Organizer
      Primate Society of Japan
    • Place of Presentation
      Kyoto University, Clock Tower Centennial Hall Building
    • Year and Date
      2015-07-18 – 2015-07-20
  • [Presentation] First report on the white morph western purple-faced leaf langur (Trachypithecus vetulus nestor) in Sri Lanka2015

    • Author(s)
      C.A.D. Nahallage, M.A. Huffman, R. Kumara
    • Organizer
      Primate Society of Japan
    • Place of Presentation
      Kyoto University, Clock Tower Centennial Hall Building
    • Year and Date
      2015-07-18 – 2015-07-20
    • Invited

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Published: 2017-01-06  

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