The study of functional genomics in soybean by FOX hunting system

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: University of Miyazaki
• Principal Investigator: Akashi Ryo 宮崎大学, 農学部, 教授 (20253809)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: FOXハンティングシステム / ファンクショナルゲノミックス / 西洋ミヤコグサ / SYNC1遺伝子 / スーパールート / ダイズ

Outline of Final Research Achievements

In this study, we explored plant biomass and seed yield-related genes, and identified the gene functions by using Lotus corniculatus FOX-superroot lines (FOXSR lines) randomly transformed with Arabidopsis thaliana cDNAs. Further, we transformed the candidate gene, an asparaginyl-tRNA synthetase gene (SYNC1), into soybean and verified its functions in this typical legume crop, soybean. FOXSR lines harboring the SYNC1 gene increased in plant height and branch node number. Similarly, transgenic soybeans which were highly regulated by SYNC1 gene obtained the same results with those of the FOXSR lines. Moreover, high expression of SYNC1 gene affected amino acid synthesis in soybean seeds. Therefore, SYNC1 gene is considered to be effective in increasing plant biomass and seed yield, and improving amino acid contents in legume crops.

Free Research Field

植物遺伝育種学