Research of MD-2 protein in rice innate immunity system mediated by OsRac1.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Keio University (2015-2016); Nara Institute of Science and Technology (2014)
• Principal Investigator: FUJIWARA Masayuki 慶應義塾大学, 政策・メディア研究科(藤沢), 特任助教 (70403350)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: イネ / 植物免疫 / 分泌タンパク質 / 相互作用 / 質量分析 / プロテオーム

Outline of Final Research Achievements

OsRac1, a small GTPase in rice, is involved in regulation of NADPH oxidase producing reactive oxygen species during oxidative burst and initiation of cell death during innate immune responses. Recently, we have tried to isolate OsRac1-interacting proteins by various methods. These results revealed the signaling network of OsRac1, named the Defensome network, which is composed of immune receptors. In this study, we isolated MD-2-related lipid recognition proteins as OsRac1 interacting proteins by immunoprecipitation and mass spectrometric analysis. This interaction were found only in constitutively active OsRac1 rice cultured cells, not found in negatively active OsRac1. Our studies revealed that MD-2 protein related cell death and secreted outside of the cell by pathogen stimulus. Fourthermore we found that MD-protein interact with phosphorylation related proteins.

Free Research Field

植物病理