2016 Fiscal Year Final Research Report
Studies on the status and origin of proteineous materials in soil
Project/Area Number |
26450072
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant nutrition/Soil science
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Research Institution | Hirosaki University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 土壌有機物 / タンパク質 / 土壌微生物 / 赤外線吸収スペクトル / 三次元蛍光スペクトル |
Outline of Final Research Achievements |
The objective of this study was to characterize the proteineous materials in soil and to elucidate the origin of the proteinous materials. When soils were fractionated by specific gravity and aggregate-size and analyzed by infrared absorption spectroscopy, proteineous materials were mainly present as mineral-associated forms. Alkaline extraction and three-dimensional excitation-emission matrix fluorescence spectroscopy indicated that the proteineous materials in soil were mostly not bound to humic substances. The amounts of proteins extracted during the decomposition process of organic matter in the soil showed a significant positive correlation with the soil microbial biomass. In addition, parts of the extracted proteins were identified as a bacterial extracellular enzyme. These results suggested that a major source of proteineous materials in soil was extracellular proteins produced by soil microorganisms.
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Free Research Field |
土壌学
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