2016 Fiscal Year Final Research Report
Challenges for the improvement of photosynthesis and plant growth by multiple overexpression of enzymes of the Calvin-Benson cycle in rice
| Project/Area Number |
26450074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Iwate University (2016)
Tohoku University (2014-2015) |
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Principal Investigator |
Suzuki Yuji 岩手大学, 農学部, 准教授 (80374974)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 光合成 / 炭素同化 / カルビンサイクル / 遺伝子組換え / イネ |
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| Outline of Final Research Achievements |
The Calvin-Benson cycle enzymes Rubisco and transketolase were co-overproduced in rice in order to enhance photosynthetic performance and plant growth. The mRNA levels of these genes were increased in transgenic plants with their co-overexpression. The protein levels of Rubisco and transketolase on a leaf area basis were increased by 35 to 53% and 39 to 84%, respectively. However, the rates of CO2 assimilation were similar to those in wild-type plants. Thus, co-overproduction of Rubisco and transketolase did not lead to enhancement of photosynthetic performance via improvement of the Calvin-Benson cycle metabolism in rice.
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| Free Research Field |
植物栄養学
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