Histochemical and cytochemical approaches toward elucidating the mechanisms of functional expression of barley high-affinity nitrate transport system

2017 Fiscal Year Final Research Report

• Project/Area Number: 26450076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Niigata University
• Principal Investigator: Sueyoshi Kuni 新潟大学, 自然科学系, 教授 (10216278)
• Research Collaborator: 野澤 大樹 ; 高田 雅大
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 硝酸イオン / トランスポーター / オオムギ

Outline of Final Research Achievements

In the present study, an interaction between two proteins (HvNRT2.1 and HvNAR2.3), both of which configure the high-affinity nitrate transport system in barley roots, was investigated histochemically and cytochemically. These two proteins localize on the plasma membrane of the outermost cell layer in the barley root cortex. In addition, these proteins interact on the plasma membrane as revealed by BiFC analysis. When HvNRT2.1 and HvNAR2.3 were expressed individually in epidermal cells of onion, each protein failed to localize the plasma membrane. These results indicate that these two proteins may need each other for localizing on the plasma membrane.

Free Research Field

植物栄養学