The role of nucleoid structure in bacterial transcriptional regulation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Oshima Taku 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50346318)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Kaneyoshi 法政大学, 生命科学部, 教授 (40351580) ; ISHIKAWA Shu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (30359872)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ChIP-seq / GeF-seq / 大腸菌 / 核様体 / H-NS / HU / Fis / IHF

Outline of Final Research Achievements

We performed the modified high resolution ChIP-seq analysis of E. coli nucleoid proteins to identify the binding regions of nucleoid proteins in base pair resolution. The results indicated that some nucleoid proteins recognize specific sequences at over 3000 regions in E. coli genome, while we could not find the specific recognition sequences of H-NS and HU. Our results also suggested that each nucleoid protein may independently interact with genomic DNA. However, because the independent interaction of each nucleoid protein affects to the functions of other nucleoid proteins, there is the inter-relation of nucleoid proteins to form functional nucleoid structure in E. coli cells.

Free Research Field

ゲノム微生物学