Elucidation of molecular mechanisms on the stress response in oleaginous microorganism thraustochytrid and its application to value-added lipid production

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450092
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hiroshima University
• Principal Investigator: Aki Tsunehiro 広島大学, 先端物質科学研究科, 教授 (80284165)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 脂質生合成 / ラビリンチュラ / オーランチオキトリウム / カロテノイド / 遺伝子発現調節 / 酵素機能

Outline of Final Research Achievements

This research aims to elucidate the molecular mechanisms underlying the stress response nature of carotenoid production which has been suggested in transcriptome of marine thraustochytrid, Aurantiochytrium. Analysis on the functional domain disruptants of β-carotene synthase CrtIBY did not suggest any cooperative action between three domains. A luciferase reporter system was constructed to investigate the regulation mechanisms of gene expression of CrtIBY. This system reproduced the expression pattern of CrtIBY gene, being enhanced at the stationary and starvation phases of cultivation.

Free Research Field

応用微生物学