Biochemical analysis of oxidative fermentation of novel oxidized sugars having an intramolecular ketone

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Yamaguchi University
• Principal Investigator: ADACHI OSAO 山口大学, その他部局等, 名誉教授 (20027189)
• Co-Investigator(Kenkyū-buntansha): 赤壁 善彦 山口大学, 創成科学研究科, 教授 (20274186)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 酸化発酵 / 酢酸菌 / 分子内ケトンをもつ酸化糖 / 5-ケトプシコース / 4-ケトペント酸 / Ga. liquefaciens

Outline of Final Research Achievements

A novel oxidation of pentonates to 4-keto- pentonates was analyzed with Gluconobacter thailandicus NBRC 3258. Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca2+. The enzyme oxidized pentonates to 4-keto-pentonates at the optimum pH of 4.0. In addition, the enzyme oxidized fructose to 5-keto- fructose, psicose to 5-keto-psicose, including the other polyols such as, glycerol, ribitol, arabitol, and sorbitol. Thus, pentonate 4-dehydrogenase was found to be identical with glycerol dehydrogenase (GLDH). The reaction versatility of quinoprotein GLDH was notified in this study.　Oxidative fermentation of hydroxypyruvate　was examined and two oxidizing systems have been indicated:　System 1: Glycerol - Dihydroxyacetone - Hydroxypyruvic aldehyde - Hydroxypyruvic acid.　System 2: Glycerol - Glyceric acid - Hydroxymalonaldehyde -　Hydroxymalonic acid

Free Research Field

応用微生物学