2016 Fiscal Year Final Research Report
Genetic engineering on increasing NADPH supply for reductive biotransformation by acetic acid bacteria.
| Project/Area Number |
26450095
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 酢酸菌 / 発酵 / 応用微生物 / 酵素 / バイオテクノロジー |
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| Outline of Final Research Achievements |
Gluconobacter sp., acetic acid bacterium, oxidizes glucose on the outer surface of the cytoplasmic membrane with membrane-bound glucose dehydrogenase (GdhM) and accumulates gluconic acid and its derivatives. Thus, only limited parts of glucose are transported into the cytoplasm and metabolized. Because glucose metabolism in the cytoplasm mostly depends on pentose phosphate pathway, it is reasonably assumed that G. oxydans likely produces much NADPH, if high amounts of glucose are metabolized in the cytoplasm. This study aimed to increase NADPH supply from glucose by inactivating GdhM. This study tried to couple NADPH produced in the ΔgdhM cells and the reduction reaction producing shikimate from dehydroshikimate. When cultivated on glucose, biomass yield and amounts of an end metabolite in the ΔgdhM strain were elevated. Taken together with enzyme activity measurements, some changes on glucose metabolism in the ΔgdhM cells suggested increases in the NADPH level.
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| Free Research Field |
生化学
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