2016 Fiscal Year Final Research Report
Development of glycosides synthesis system using a novel enzymatic activation of carbohydrates with enzymes
| Project/Area Number |
26450114
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 糖転移反応 / フラクトシル化 / スクロース |
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| Outline of Final Research Achievements |
The purpose of this study is to make donor substrates for transglycosylation, which glycosidases catalyze. Using β-fructofranosidase and levansucrase, I succeeded in transferring a fructosyl group of sucrose to each saccharide, such as xylose, galactose, mannose, isomaltooligosaccharides, maltooligosaccharides, and glucuronic acid. The reaction product can be a donor substrate of the transglycosylation by glycosidases. Furthermore, I investigated the transglycosylation properties of several glycosidases and modified their specificity through molecular analyses. In addition, nucleotide sugar was successfully synthesized by the reaction of sucrose synthase with fructosyl saccharide as substrate.
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| Free Research Field |
応用生物化学
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