2017 Fiscal Year Final Research Report
Three-dimensional Structure of Chemical Sensor Keap1
| Project/Area Number |
26450115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | センサータンパク質 / 酸化ストレス |
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| Outline of Final Research Achievements |
In this research the molecular mechanism of oxidative stress response by human cell are analyzed. In order to unveil the molecular mechanism in which the sensor protein Keap1 recognize the oxidative stress and various toxic chemicals, I prepared the recombinant Keap1 protein for the crystal analysis. The crystallization of Keap1 mutants was succeeded, and X-ray diffraction analysis was performed by using the synchrotron X-ray source performed X-ray diffraction experiment. The method to detect the intercalations between Keap1 and the toxic chemicals from vegetables was developed.
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| Free Research Field |
構造生物学
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