Generation and transfer of disulphide bonds in plant cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Ehime University (2015-2016); Yamagata University (2014)
• Principal Investigator: Yayoi Onda 愛媛大学, 農学研究科, 准教授 (70368463)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 植物 / 酵素 / ジスルフィド結合

Outline of Final Research Achievements

In the developing endosperm cells of rice (Oryza sativa), disulphide bonds enable redox control of protein packaging into the protein storage organelles, thus providing nutritional reserves for the next generation. In this study, we aimed at unravelling the mechanisms that modulate the redox pathways for disulphide bond formation, which generate hydrogen peroxide as a by-product. The pathways for disulphide bond formation require de novo disulphide-generating enzyme (e.g. ERO1) and disulphide carrier protein (e.g. PDI) in the endosperm cells of rice. A potential function of the QSOX family member, which catalyses both de novo disulphide generation and disulphide transfer, is discussed.

Free Research Field

植物生化学・分子細胞生物学