Elucidation of the reaction mechanism of plant flavonoid C-glucosyltransferases

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Shinshu University
• Principal Investigator: TAGUCHI Goro 信州大学, 学術研究院繊維学系, 准教授 (70252070)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: C-配糖化酵素 / フラボノイドC-配糖体 / 酵素反応解析 / Ｘ線結晶構造解析

Outline of Final Research Achievements

In this project, we identified C-glycosyltransferases those are responsible for the biosynthesis of valuable flavonoid C-glycosides from several plant species, such as buckwheat, citrus and leguminous plants. We characterized the enzymatic reaction of them precisely, and also analyzed the crystal structure of the protein to elucidate the reaction mechanisms of the C-glycosyltransferases producing carbon-carbon bonds between the sugar moiety and the aromatic carbon of flavonoids. We also achieved the production of C-glucosides of flavonoid by bioconversion using Escherichia coli expressing C-glucosyltransferases.

Free Research Field

植物生化学