Study on mechanisms of metabolic regulation and structure-function relationship of sphingolipids in budding yeast

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyushu University
• Principal Investigator: Tani Motohiro 九州大学, 理学研究院, 准教授 (20452740)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: スフィンゴ脂質 / 複合スフィンゴ脂質 / セラミド / 出芽酵母 / ストレス応答

Outline of Final Research Achievements

The aim of this study is to understand the relationship between structural diversity and biological function of complex sphingolipids in yeast Saccharomyces cerevisiae. Results of the research was the followings: (1) it was found that MIPC, one of the subtypes of complex sphingolipid in yeast, is important for maintenance of functions of cell wall, (2) change in activity of serine palmitoyltransferasae affects sensitivity to Syringomycin E, a toxin produced by the plant phathogenic bacterium, (3) downregulation of de novo sphingolipid biosynthesis rescues functional defects caused by deletion of RVS167 and RVS161 encoding the amphiphysin family proteins, (4) deletion of vacuolar H+-ATPase (V-ATPase) causes dramatic alteration of complex sphingolipid composition, and it was suggested that the alteration is an adaptation mechanism for defect of intracellular pH homeostasis.

Free Research Field

脂質生化学