Elucidaton of molecular mechanism of remodeling for cardiolipin

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450139
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Kyoto University
• Principal Investigator: Masato Abe 京都大学, 農学研究科, 助教 (30543425)
• Co-Investigator(Renkei-kenkyūsha): OKU Masathide 京都大学, 大学院農学研究科, 助教 (10511230)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: カルジオリピン / リン脂質 / リモデリング / tafazzin / アシルトランスフェラーゼ / ミトコンドリア / ホスファチジルコリン / リゾカルジオリピン

Outline of Final Research Achievements

Remodeling of the acyl chains of cardiolipin (CL) is responsible for final molecular composition of mature CL after de novo CL synthesis in mitochondria. In light of the diversity of the acyl compositions of mature CL between different organisms, the mechanism underlying tafazzin-mediated transacylation remains to be elucidated. We investigated the mechanism responsible for transacylation using purified S. cerevisiae tafazzin with liposomes composed of various sets of acyl donors and acceptors. The results revealed that tafazzin efficiently catalyzes transacylation in liposomal membranes with highly ordered lipid bilayer structure. Tafazzin elicited unique acyl chain specificity against phosphatidylcholine (PC) as follows: linoleoyl (18:2) > oleoyl (18:1) _ palmitoleoyl (16:1) >> palmitoyl (16:0). In these reactions, tafazzin selectively removed the sn-2 acyl chain of PC and transferred it into the sn-1 and sn-2 positions of MLCL isomers at equivalent rates.

Free Research Field

生物有機化学