Identification of acylhomoserine lactone synthases by affinity beads

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: Kai Kenji 大阪府立大学, 生命環境科学研究科, 講師 (40508404)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: クオラムセンシング / アシルホモセリンラクトン / ケミカルバイオロジー / アフィニティービーズ

Outline of Final Research Achievements

Bacteria communicate using chemical signals to sense cell density and regulate diverse coordinated behaviors by a process known as quorum sensing (QS). N-Acylhomoserine lactones (AHLs), one class of QS signals in Gram-negative bacteria, are synthesized from the acyl-acyl carrier protein (acyl-ACP) and
S-adenosylmethionine (SAM) by LuxI-type synthases. We here report the affinity purification of AHL synthases using beads conjugated with an enzyme inhibitor, which was designed based on the catalytic intermediate acyl-SAM. This is the first study to show that QS signal synthases could be purified by a ligand-based affinity
protocol.

Free Research Field

生物有機化学