Search for metallo-beta-lactamase inhibitors produced by microorganisms

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Kitasato University
• Principal Investigator: Shiomi Kazuro 北里大学, 感染制御科学府, 教授 (40235502)
• Co-Investigator(Renkei-kenkyūsha): ASAMI Yukihiro 北里大学, 大学院感染制御科学府, 助教 (70391844) ; MATSUMOTO Atsuko 北里大学, 大学院感染制御科学府, 准教授 (20300759) ; HANAKI Hideaki 北里大学, 大学院感染制御科学府, 教授 (60286747)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 抗生物質 / 薬剤耐性 / 天然物化学 / 微生物化学

Outline of Final Research Achievements

We screened more than 10,000 cultured broths of actinomycetes and fungi that showed metallo-beta-lactamase (MBL) inhibition activity using MBL-producing clinically isolated strains of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. The paper disc method with or without meropenem was used for the screening, and we selected samples that displayed larger inhibition zone in the presence of meropenem. Large-scale culture was carried out for the selected samples, and we isolated the MBL inhibitory compounds from cultured broths of a Penicillium strain and a Paecilomyces strain. Though the compounds were known ones, their MBL inhibition activity has not been reported. Both compounds inhibited IMP-1 type MBL at the IC50 values of 20 μg/ml.

Free Research Field

天然物化学、微生物化学