Development of new channelrhodpsin having the long conjugated chromophore

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: WADA Akimori 神戸薬科大学, 薬学部, 教授 (80158683)
• Co-Investigator(Renkei-kenkyūsha): SHICHIDA Yoshinori 京都大学, 大学院・理学研究科, 教授 (60127090)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: チャネルロドプシン / 発色団 / 長い共役系 / 赤色光 / レチナール / ケミカルバイオロジー

Outline of Final Research Achievements

Optogenetics is a powerful new tool, which allows control of neuronal activity by light. Channelrhodopsins are light-gated cation channels and have been used for optogenetics. In order to construct a new type of channelrhodopsin having the chromophore responding to the longer wavelength, we prepared the novel retinal analogs with double bond-elongated at the Schiff base, side chain or cyclohexene ring of retinal and 3,4-dehyrdoretinal.All analogs synthesized here bound to apo-protein to afford the new channelrhodopsins. Although the red shift values of ramuda max were only 10-20 nm, these channelrhodopsins exhibited the half of absorption maximum at about 560-580 nm, which were about 50-80 nm red shifted than that of native channelrhodopsin.

Free Research Field

生物有機化学