Flow-injectin ESR study for superoxide radical scavenging activity of antioxidants of vegetables

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Tajima Kunihiko 京都工芸繊維大学, 分子化学系, 教授 (50163457)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: スーパーオキシドラジカル / アルコキシルラジカル / 抗酸化活性 / 青果物 / 流通型ESR装置

Outline of Final Research Achievements

A new flow-injection ESR (FI-ESR) systems special for quantitative spin-trapping ESR detection of　alkoxyl (RO) radicals were developed to evaluate the alkoxy radical elimination capacity (AREC) of plants derived potent antioxidants. The AREC system was enable to observe the DMPO (5,5-dimethyl-1-pyrroline N-oxide) spin adducts of the alkoxyl radical produced by thermal decomposition of AAPH (2,2'-azobis(2,4-amidinopropane) dihydrochloride) using the flow-injection ESR (FI-ESR) method. The AREC value of plants derived phenolic compounds, such as caffeic acid, catechine, and rutine, were determined by using Trolox (6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid, TRX) as a standard sample. The newly defined AREC value is applicable for various biosubstances, and is far more excellent and reliable indicator than the oxygen radical absorption capacity (ORAC) value determined by the well-known ORAC-fluorescein assay methods.

Free Research Field

ラジカル反応機構解析