Reproductive interference of Chamaecyparis pisifera by the heterospecific pollens from artificial plantation of Cryptomeria japonica and Chamaecyparis obtusa

2017 Fiscal Year Final Research Report

• Project/Area Number: 26450190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Gifu University
• Principal Investigator: MUKAI Yuzuru 岐阜大学, 応用生物科学部, 教授 (80283349)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 繁殖干渉 / 空中花粉 / 生殖隔離 / ヒノキ科 / サワラ / 種判別

Outline of Final Research Achievements

To verify reproductive interference in Chamaecyparis pisifera by heterospecific pollens from artificial stands of Cryptomeria japonica and Ch. obutusa, reproductive isolation among the three Cupressaceae species and species composition of airborne pollens were investigated.
The results from the withdrawal of pollination drop and post pollination behavior of the heterospecific pollen tubes in the ovules suggest that reproductive isolation among the three species did not function at the pollination stage but in the post-pollination stage.
To evaluate species composition of airborne pollen, chloroplast DNA based genetic markers and single-pollen genotyping method was developed. Cupressaceae pollens were isolated by microscope and genotyped with the markers. The 90% of the Cupressaceae pollen was identified as Ch. obutusa. These results suggest that Ch. pisifera might suffer reproductive interference due to the heavier contamination of heterospecific pollens from related species.

Free Research Field

森林遺伝学