2016 Fiscal Year Final Research Report
Elucidation of the signaling pathway to the host of basidiomycete virus and functional analysis on fruiting body formation
| Project/Area Number |
26450234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Tokyo Kasei University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ウイルス / 担子菌 / 菌類ウイルス / 遺伝子発現 / マイクロアレイ / 子実体 / シグナル / キノコ |
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| Outline of Final Research Achievements |
We isolated two mycoviruses from Grifola flondosa (MAITAKE), and finally identified two viruses as Parititivirus (GfPV1) and RNA Virus (GfRV1). GfPV1 has a capcid protein gene and RdRp protein. And we successfully to purified virions with high centrifugation method and with its sequences, finally we identified as new type of Parititivirus in Grifola flondosa. On the other hand, GfRV1 possibly has no coat proteins, thus tentatively we identified as capcidless virus. Both of them behave as no phenotypic effects as host of the G. flondosa, but treated with cell cycle arrest chemical and low temperature harvest influence its growth. And we created virus free hosts and re-infectious each viruses respectively hosts, thus we are now start to analysis of virus positive vs. virus free hosts gene expression analysis as a microarray. This results will be future study.
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| Free Research Field |
菌類ウイルス
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