2016 Fiscal Year Final Research Report
Developmental study of marine virus monitoring method in natural sea area.
| Project/Area Number |
26450249
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | The Nippon Dental University (2015-2016)
Keio University (2014) |
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Principal Investigator |
Toyoda Kensuke 日本歯科大学, 生命歯学部, 非常勤講師 (40585874)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 海洋ウイルス / 珪藻 / リアルタイムqPCR / ELISA / 定量検出 |
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| Outline of Final Research Achievements |
A monitoring method in a more sensitive and stable natural sea area for three new viruses infected with marine floating diatoms, Cylindrotheca closterium, have developed. During the experiment, host real-time qPCR, genome analysis of three new viruses, development of real-time qRT-PCR, and development of a virus quantitative detection method by ELISA were performed.
As a result, method of stable and good quantification / detection sensitivity from natural sea water by real-time qRT-PR method and Direct ELISA method have developed.
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| Free Research Field |
海洋ウイルス学
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