Transcriptional profiling of olfactory inprinting-related molecules in chum salmon brain using next-generation sequencer

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: Kudo Hideaki 北海道大学, 水産科学研究院, 准教授 (40289575)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: サケ / 母川回帰 / 嗅覚 / 刷込 / 脳 / 嗅球 / 終脳 / 次世代シーケンサー

Outline of Final Research Achievements

In this study, genes involved in olfactory imprinting (OI) and memory retrieval (OMR) were searched in the chum salmon olfactory center. Transcriptome analyses using next-generation sequencer were carried out on olfactory center of the seaward migration (OI period) juveniles and the homeward migration (OMR period) adult salmon. In each period of seaward and homeward migration, these genes indicates the minimum expression at the silent stage, and indicated expression higher than 10 fold from silent stage at active stage. In this selective condition, the same genes were not contained in both selected transcripts. The molecules of pre-synaptic vesicle exocytosis, cell-growth/differentiation, etc. were included in the OI-related candidate genes. On the other hand, some neurotransmitter receptors were included in the OMR-related candidate genes. Our results suggest that different gene groups are mainly functioning to OI and OMR in Pacific salmon.

Free Research Field

魚類生理学