Determination of core microbiota and its function in the rumen of Japanese Black cattle

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450371
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Hokkaido University
• Principal Investigator: Koike Satoshi 北海道大学, 農学研究院, 准教授 (90431353)
• Research Collaborator: ENDO Tetsushiro 北海道立総合研究機構, 畜産試験場
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 黒毛和種 / ルーメン発酵 / ルーメン細菌 / 菌叢解析

Outline of Final Research Achievements

In this study, we aimed to determine the core microbiome and its function in the rumen of JB cattle. MiSeq analysis of the microbiota revealed that Lachnospiraceae, Prevotellaceae, Ruminococcaceae, unclassified Bacteroidales and unclassified Clostridiales occupied more than 70% of rumen microbiota in JB cattle. Therefore, these five phylotypes were considered as core members of the microbiota, and we attempted to isolate starch degrading bacteria belonging to these phylotypes. Among 178 newly isolated strains, 12 strains were classified into either of target phylotypes; 5 strains for Lachnospiraceae, 5 strains for Ruminococcaceae and 2 strains for Prevotellaceae. Under different pH conditions, Lachnospiraceae strain grew at pH 5.5; meanwhile, strains of Prevotellaceae and Ruminococcaceae did not.
Therefore, the strains belonging to core phylotypes of rumen microbiota in JB cattle might be involved in feed digestion in different manners.

Free Research Field

家畜栄養学、消化管微生物学