Immunological analysis of suppression mechanism of immediate hypersensitivity by infection of Trichinella pseudospiralis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450401
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: Nagano Isao 岐阜大学, 大学院医学系研究科, 准教授 (40283296)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 旋毛虫 / 即時型アレルギー / 免疫抑制 / 分泌タンパク質 / サイトカイン / 組換えタンパク質 / スギ花粉

Outline of Final Research Achievements

It is proposed that Trichinella infection or Trichinella-derived molecules（Tp53）modulate host’s immunity.
In this study, we investigated the effect of Trichinella infection or Tp53 on immediate hypersensitivity in mice sensitized with Japanese cedar pollen. As a result, trichinella infection or Tp53 administration attenuated inflammatory reactions in the lungs, and production of specific IgE, IgG2a, IgG2b antibody decreased. In addition, production of IL-4 and IL-17 in infected mouse spleen cells or splenocytes treated with Tp53 was suppressed. And our observation showed that the Tp53 suppressed NF-κB signal pathway of macrophage, as indicated by the results of inhibition of LPS stimulation-induced NF-κB p65 nuclear translocation, phosphrylation of NF-κB signal proteins (IKK, p65 and IκBα) and degradation of IκBα. The results suggest that the Tp53 protein may modulate host immune response by inhibiting macrophage activation via specifically affecting NF-κB signal pathway.

Free Research Field

獣医学　寄生虫学