Mechanistic study of the role of MUC1-like molecule in malignant proliferation of canine hemangiosarcomas

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450426
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: Sakai Hiroki 岐阜大学, 応用生物科学部, 准教授 (40283288)
• Research Collaborator: Shehata Ibrahim Anwar
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 血管肉腫 / 犬 / Peroxiredoxin 6 / RNA干渉 / 培養細胞 / アポトーシス

Outline of Final Research Achievements

Immunohistochemically, canine hemangiosarcomas were strongly positive using commercially provided anti human MUC1 antibody. In canine hemangiosarcoma cell lines, lesser molecular weight band (22kDa) was detected with Western blotting. Peptide sequencing after immunoprecipitation with the antibody revealed the antigen was canine peroxiredoxin 6(Prdx6). Apoptosis was induced in canine hemangiosarcoma cell lines by Prdx6 knockdown with small interfering RNA method. uPA/uPAR, which associating with Prdx6 in malignant proliferation and invasion, were strongly positive in canine hemangiosarcomas. In conclusion, Prdx6 and uPA/uPAR may associate with in malignant proliferation of canine hemangiosarcomas.

Free Research Field

獣医病理学