Etiology of boar spermatozoa affected with summer infertility in an attempt to develop a novel therapy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450427
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: Murase Tetsuma 岐阜大学, 応用生物科学部, 教授 (30303514)
• Research Collaborator: SHIMIZU Kenji
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ブタ / 夏季不妊症 / 精子 / 人工授精 / 体外受精 / 先体反応

Outline of Final Research Achievements

It was considered that one of the mechanisms underlying the hypersensitive induction of the acrosome reaction may be due to an increased content of cAMP [cAMP]i in boar spermatozoa collected during summer. Addition of cholesterol or removal of bicarbonate in the semen extender did not affect [cAMP]i of spermatozoa. Thus, a mechanism that cholesterol delays the induction of the acrosome reaction may be the effect on the generation of cAMP during the process of the acrosome reaction raher than during storage. Addition of magnesium lowered the occurrence of the acrosome reaction. The staining method of sperm acrosomes with FITC-PNA was modified. Preincubation of boar spermatozoa with adipose-derived stem cells (ADSC) increased the ability of in vitro fertilizing ability. Cholesterol, magnesium and ADSC are prospective in applying to a 'therapy of boar spermatozoa' suffering from summer infertility.

Free Research Field

獣医臨床繁殖学