2016 Fiscal Year Final Research Report
Monitoring for multiple contamination of mycotoxins in feeding environment of cattle herds
| Project/Area Number |
26450429
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Yamaguchi University (2015-2016)
Kagoshima University (2014) |
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Principal Investigator |
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| Research Collaborator |
Johanna Fink-Gremmels ユトレヒト大学, 獣医学部, 教授
KOKUSHI EMIKO 鹿児島大学, 水産学部, 助教
NAKAMURA MASAYUKI 鹿児島大学, 農学部, 准教授
MIYAMOTO AKIO 帯広畜産大学, 畜産学研究科, 教授
DEGUCHI EISABURO 鹿児島大学, 共同獣医学部, 教授
HASUNUMA HIROSHI (有)シェパード中央家畜診療所
FUSHIMI YASUO (有)シェパード中央家畜診療所
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ウシ / ゼアラレノン / ステリグマトシスチン / 尿 / 繁殖性 |
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| Outline of Final Research Achievements |
We investigated the effects of exposure to zearalenone levels on the anti-Mullerian hormone (AMH) endocrine levels of cattle. Urinary zearalenone and its metabolites concentrations were measured by chromatography-tandem mass spectrometry (LC/MS/MS), and serum AMH concentrations were determined. The results indicate that zearalenone intake from dietary feed, even when below the threshold zearalenone contamination level permitted in Japan, may affect the ovarian antral follicle populations of postpartum cows. Additionally, we determine the levels of the fungal toxin sterigmatocystin (STC) in the urine of cattle using LC/MS/MS. The results indicate that STC is extensively conjugated in the liver, presumably to glucuronic acid, and confirmed co-existing contamination of both ZEN and STC in the dietary feeds of cattle. Overall, mycotoxins in urine samples are useful biological markers for monitoring the systemic exposure of cattle to multiple mycotoxins.
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| Free Research Field |
獣医繁殖学、産業動物獣医学
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