2017 Fiscal Year Final Research Report
Study on Pathophysiology of cattle inflammatory diseases as an indicator of endotoxin activity value and establishment of rapid prognostic diagnosis
| Project/Area Number |
26450431
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
樋口 豪紀 酪農学園大学, 獣医学群, 教授 (00305905)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | エンドトキシン / ウシ / Cow Side Test / 予後判定 / 甚急性乳房炎 / 牛呼吸器病症候群 |
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| Outline of Final Research Achievements |
The aim of the present study was to compare endotoxin activities detected in raw milk samples obtained from cattle by a commercially available portable test system (PTS) and traditional microplate limulus amebocyte lysate (LAL) kinetic turbidimetric (KT) assay. Raw milk samples were obtained from 65 dairy cattle without and with clinical mastitis, respectively. The endotoxin activities detected in 200- or 400-fold diluted milk samples were similar between PTS and KT assay. The results obtained from 200- (r2=0.778, p<0.001) and 400-fold diluted milk samples (r2=0.945, p<0.001) using PTS correlated with those using KT assay. The median milk endotoxin activities in Gram-positive and Gram-negative clinical mastitis cows were 0.655 and 11,523.5 EU/ml, respectively. The results of the present study suggest that PTS as a simple and easy test to assess endotoxin activity in raw milk is efficient, simple and reproducible.
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| Free Research Field |
生産動物外科学
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