Fundamental study for the development of new radiosensitizers based on the molecular mechanism of DNA double strand break repair of companion animals

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450438
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: National Institutes for Quantum and Radiological Science and Technology
• Principal Investigator: KOIKE AKI 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 加速器工学部, 技術員(任非) (50415410)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 放射線 / 伴侶動物 / 増感剤 / 犬 / Ku70 / NHEJ / XLF / XRCC4

Outline of Final Research Achievements

Understanding the molecular mechanisms of DNA double-strand break (DSB) repair is important for developing new radiosensitizers for companion animal cancers. Radiation- induced DSBs are mainly repaired by nonhomologous end joining (NHEJ) mechanisms in mammalian cancer cells. Therefore, it is important to clarify the molecular mechanisms of underlying NHEJ in order to get valuable information for the development of potential molecular targets for new radiosensitizers in the treatment of canine cancers. On the other hand, cDNAs of core NHEJ factors of canine species have not been cloned. Furthermore, the sequence, localization and control mechanisms of core NHEJ factors of canine species have not been published.
In this study, we performed fundamental study for the development of new radiosensitizers based on the molecular mechanism of Ku-dependent NHEJ of canine species.

Free Research Field

放射線生物学