2016 Fiscal Year Final Research Report
Elucidation of the structure and function of a novel chicken serum protein
| Project/Area Number |
26450448
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ニワトリ / 血清 / タンパク質 / スカベンジャー受容体システインリッチドメイン |
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| Outline of Final Research Achievements |
EW135 is a chicken egg white protein with 9 tandem repeats of group B scavenger receptor cysteine rich (SRCR) domain. We previously discovered a chicken serum protein named CS120 which reacts with anti-EW135 antibodies. The purpose of this project was to elucidate the structure and function of CS120. We developed a method for the purification of CS120 by using ion-exchange and affinity chromatographies. By cDNA cloning, the primary structure of CS120 has been determined. CS120 was found to consist of 8 tandem repeats of group B SRCR domain. CS120 bound to Staphylococcus aureus and protein A, while it did not show an anti-influenza virus activity.
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| Free Research Field |
免疫学
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