2016 Fiscal Year Final Research Report
Involvement of s-nitrosylation and nitric oxide on apoptosis in rat uterus
| Project/Area Number |
26450451
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Integrative animal science
|
|---|
| Research Institution | Azabu University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Takehito 麻布大学, 獣医学部, 准教授 (90532052)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 一酸化窒素(NO) / S-ニトロシル化タンパク質 / アポトーシス / 子宮 / ラット |
|---|
| Outline of Final Research Achievements |
We examined the role of nitric oxide (NO) on apoptosis and S-nitrosylation that might affect apoptosis. To inhibit NO production, pregnant rats were administered an NO synthase inhibitor, L-NAME, apoptotic cells were observed in the endometrium of L-NAME-treated and -untreated rats. L-NAME-treatment significantly increased apoptosis and the expressions of cleaved-caspase-3 and X-linked inhibitor of apoptosis (XIAP), but significantly decreased thioredoxin (Trx) -1. Furthermore, L-NAME-treatment significantly induced S-nitrosylation (SNO) of cleaved caspase-3 and XIAP that were associated with a marked decline in Trx-1. These results suggest that apoptosis in the endometrium is positively and negatively regulated by NO through caspase and its inhibitor and S-nitrosylation of those factors during mid-gestation.
|
| Free Research Field |
基礎獣医学・基礎畜産学
|
