2016 Fiscal Year Final Research Report
Suppression mechanism of sperm cell death with lactoferrin mediated by TLR
| Project/Area Number |
26450456
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ラクトフェリン / ラクトフェリシン / TLR / アポトーシス / 常在細菌 / 胚移植 / 胚着床 / 精子 |
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| Outline of Final Research Achievements |
Experiments were designed to elucidate the mechanism of TLR mediated spermatogenesis by lactoferrin. We used mouse sperm as a material, co-cultured with bacterial cell component and lactoferrin, then examined cells induced to undergo apoptosis using immunohistological method, and analyzed the expression of genes related to apoptosis by biochemical method and examined. As a result, it was revealed that lactoferrin suppresses sperm apoptosis by bacteria and does not affect fertility and embryogenesis. From the above, it became clear that spermatogenesis and cell death caused by bacteria by lactoferrin lactoferricin were suppressed, and it was revealed that fertilization rate and implantation rate were improved.
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| Free Research Field |
実験動物学
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