Molecular mechanisms of ABC transporter, Bcrp1, in the oxidative stress response in mouse embryonic stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450460
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Kindai University
• Principal Investigator: MITANI Tasuku 近畿大学, 先端技術総合研究所, 教授 (10322265)
• Co-Investigator(Renkei-kenkyūsha): HARATA Masahiko 東北大学, 大学院・農学研究科, 准教授 (70218642)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ES細胞 / ABCトランスポーター / 酸化ストレス

Outline of Final Research Achievements

ABC transporter, Bcrp1, defines the side population (SP) cell phenotype and seems presumptive functional regulator of stem cells. This study examined the function of Bcrp1 on the oxidative stress response of mouse ES cells. The results are summarized as follows: (1) transcriptional activator of Bcrp1 mRNA isoform specific to ES cells, (2) generation of SP cell subpopulation induced by Bcrp1 expression in ES cells, (3) induction of Bcrp1 expression and maintenance of pluripotent status by the oxidative stress response, and (4) visualization of the spatial arrangement of nuclear organization using 3D-FISH in early mouse embryos and ES cells.

Free Research Field

農学