2016 Fiscal Year Final Research Report
Study on the post-transcriptional regulation of mRNA by the insect virus proteins.
Project/Area Number |
26450467
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Insect science
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Research Institution | Kyoto Institute of Technology |
Principal Investigator |
Kotani Eiji 京都工芸繊維大学, 応用生物学系, 准教授 (10273541)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | カイコ / バキュロウイルス / タンパク質発現 / 遺伝子組換え |
Outline of Final Research Achievements |
Bombyx mori nucleopolyhedrovirus nucleic acid-binding protein BRO family likely supports the regulation of mRNA. We have shown that BRO-B and BRO-E associate with cellular TIA-1 homolog (BmTRN-1), a translational regulator in silkworm cells during viral infection. Co-expression of BRO-B and BRO-E synergistically led to a significant decrease in protein synthesis from a designed transcript carrying the 5’UTR of a cellular mRNA with no significant change of the transcript abundance. BRO family proteins were also shown to be involved in the regulation of protein synthesis by the study using the transgenic silkworms with middle silk glands expressing the BRO-A that influenced the sericin cocoon production of the silkworms. These results suggest that the BRO family could regulate protein synthesis in the silkworm individuals and the application to obtain the silkworms with a useful trait.
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Free Research Field |
Insect molecular biology
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