DNA barcoding of Scarabaeoidea for identification of agricultural pests and species diversity management

2017 Fiscal Year Final Research Report

• Project/Area Number: 26450470
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: Kyushu University
• Principal Investigator: Hosoya Tadatsugu 九州大学, 持続可能な社会のための決断科学センター, 准教授 (90467944)
• Research Collaborator: YOSHIZAWA Satoshi ; KAKIZOE Showtaro ; SUZUKI Ryoga
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: DNAバーコーディング / コガネムシ上科甲虫 / 農業害虫 / 同定 / 種多様性 / 保全管理 / 系統地理

Outline of Final Research Achievements

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. The most commonly used barcode region for animals is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase subunit I (COI). This study reports on the DNA barcoding of Japanese scarabaeoid beetles to enable their rapid and accurate identification for agricultural pest control, biodiversity analysis, detection of invasive species and imported pet beetle, and environmental impact assessment.
I collected and identified 319 taxa (54.6%) among 584 taxa (species & subspecies) in Japanese Scarabaeoid beetles. I determined sequence of 294 individuals in 52 taxa. In addition, I established "JScaBOL DNA Barcode Database of Japanese Scarabaeoid Beetles" and accumulated the information of DNA Barcode data and specimen data in this database.

Free Research Field

昆虫地理学