2016 Fiscal Year Final Research Report
Diversity, activity, and cultivation of anaerobic methane-oxidizing archaea in sediments of a shallow river estuary
| Project/Area Number |
26450478
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental agriculture(including landscape science)
|
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 嫌気的メタン酸化古細菌 / mcrA遺伝子 / dsrAB遺伝子 / 垂直分布 / 多様性 / 汽水域堆積物 / 難培養微生物 |
|---|
| Outline of Final Research Achievements |
Anaerobic methanotrophic archaea (ANME) and sulfate-reducting bacteria (SRB) are considered to be important microbes in marine sediments, since they would consume most of greenhouse gas (CH4) thorough anaerobic oxidation of methane (AOM) coupled with sulfate reduction. To date, relatively few studies regarding ecophysiology of the ANME in estuary sediments. In this study, I investigated vertical distribution, diversity, activity, and cultivation of ANME in sediments of shallow water estuary. Stable isotope labeling experiments indicated that the highest AOM activity was observed in the deepest sediment. The activity was suppressed in the presence of methanogen- or sulfate reducing bacteria specific inhibitors. The clone analysis of mcrA gene indicated the possibility that the different ANME community would be developed depending on the sediment depth.
|
| Free Research Field |
微生物学、微生物生態学
|
