2016 Fiscal Year Final Research Report
Analysis of retrograde signal via 12-oxo-phytodienoic acid
| Project/Area Number |
26450503
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Tottori University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 12-オキソフィトジエン酸 / オキシリピン / レトログレードシグナル / レドックス / 熱ショック転写因子 |
|---|
| Outline of Final Research Achievements |
To investigate the involvement of 12-oxo-phytodienoic acid (OPDA)in the regulation of Apx2 and HsfA2 expression under high light in Arabidopsis, I analyzed using fad3/fad7/fad8 mutants which can not synthesis OPDA. However, there was no significant difference in the transcript level of Apx2 and HsfA2 between the wild-type plants and fad/3fad7/fad8 mutants at 1 h after high light irradiation, suggesting that OPDA was not involved in these induction within 1 hour. RNA-seq analysis showed that the transcript levels of 239 genes were decreased (<2-fold) in the fad3/fad7/fad8 mutants compared with the wild-type plants. This finding suggests that the high light inducible expression of these genes was regulated by oxilipin containing OPDA.
|
| Free Research Field |
植物生理学
|
