Functional analysis of rice GABA shunt that responds to various abiotic stresses and its application to molecular breeding

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450504
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Shimane University
• Principal Investigator: Akama Kazuhito 島根大学, 生物資源科学部, 教授 (50252896)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: γ-アミノ酪酸 (GABA) / GABAアミノ基転移酵素 (GABA-T) / グリオキシル酸還元酵素 (GLYR) / ストレス応答 / イネ / 形質転換 / アグロバクテリウム

Outline of Final Research Achievements

In order to investigate function of gamma-aminobutyric acid (GABA) shunt in response to abiotic stresses, we established various kinds of transgenic rice lines that overexpress a gene for GABA aminotransferase (GABA -T) or glyoxylate reductase. These transgenic rice plants were subjected to abiotic stresses such as low temperature, salt, drought and hydrogen peroxide (200 mM). Temperature or drought stresses resulted in increase in biomass from most of plants. After treatment of plants with oxidative or low temperature stresses, plants were planted in the soil to cultivate for one month. The transgenic plants overexpressing OsGABA-T with RecA-chloroplst transit signal could survive at the high frequency. Free amino acid analysis of this lines demonstrated the decrease in GABA shunt-related free amino acids such as Ala, Gly, Ser, Glu and GABA, expect for Gln.

Free Research Field

植物分子生物学