Large-scale production of secondary metabolites by alteration of a transporter gene

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450506
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kanazawa Institute of Technology
• Principal Investigator: Motoaki Sano 金沢工業大学, バイオ・化学部, 教授 (80410299)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 麹菌 / トランスポーター / 二次代謝産物

Outline of Final Research Achievements

We aimed to improve the production of fungal secondary metabolites by altering a transporter gene. We chose Aspergillus oryzae, which produces many useful compounds, as the subject of our research. We identified that approximately 10% of A. oryzae transporters were not expressed at the membrane. Next, we generated and analyzed in detail many gene disruption strains of amino acid transporters in A. oryzae. Disruptions of the lysine transporter upregulated the expression of penicillin biosynthesis genes, inhibit condition of penicillin biosynthesis.
The data from these analyses will form the foundation for the mass production of a useful compound in filamentous bacteria.

Free Research Field

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