2016 Fiscal Year Final Research Report
Analysis of phosphorylation signal using a Phos-tag quencher
Project/Area Number |
26460036
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Physical pharmacy
|
Research Institution | Hiroshima University |
Principal Investigator |
Koike Tohru 広島大学, 医歯薬保健学研究院(薬), 教授 (90186586)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | プロテオミクス / 蛍光分析 / キナーゼ / フォスファターゼ / Phos-tag / リン酸化分子 |
Outline of Final Research Achievements |
By utilizing an original analytical method using Phos-tag derivatives, we have developed convenient and reliable protocols for real-time analysis of phosphorylation status of kinase or phosphatase substrates. Since the analytical volume of μM-level sample is between μL and mL, the general fluorometric apparatus can be used in a standard chemical laboratory. We believe that our Phos-tag technology will result in great progress in phophophroteomics.
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Free Research Field |
医薬分子機能科学
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