Analysis of phosphorylation signal using a Phos-tag quencher

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460036
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Hiroshima University
• Principal Investigator: Koike Tohru 広島大学, 医歯薬保健学研究院(薬), 教授 (90186586)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: プロテオミクス / 蛍光分析 / キナーゼ / フォスファターゼ / Phos-tag / リン酸化分子

Outline of Final Research Achievements

By utilizing an original analytical method using Phos-tag derivatives, we have developed convenient and reliable protocols for real-time analysis of phosphorylation status of kinase or phosphatase substrates. Since the analytical volume of μM-level sample is between μL and mL, the general fluorometric apparatus can be used in a standard chemical laboratory. We believe that our Phos-tag technology will result in great progress in phophophroteomics.

Free Research Field

医薬分子機能科学