2016 Fiscal Year Final Research Report
siRNA delivery to tumor by sequential injection of anionic polymer and siRNA lipoplex
| Project/Area Number |
26460046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | siRNA / リポソーム / 肝転移がん / コンドロイチン硫酸 / 負電荷ポリマー |
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| Outline of Final Research Achievements |
We developed novel siRNA transfer method to the liver by sequential intravenous injection of anionic polymer and cationic liposome/siRNA complex (siRNA lipoplex). When siRNA lipoplex was intravenously injected into mice bearing liver metastasis, the accumulation of siRNA was mainly observed in the lungs. In contrast, when siRNA lipoplex was intravenously injected into mice bearing liver metastasis immediately after intravenous injection of chondroitin sulfate (CS), siRNA was accumulated in tumor-metastasized liver. In terms of a gene silencing effect, sequential injections of CS plus siRNA lipoplex could reduce expression of target gene in liver metastasis. Moreover, sequential injections of CS plus siRNA lipoplex of protein kinase N3 (PKN3) siRNA could suppress tumor growth in the mice bearing liver metastasis. From these findings, sequential injection of CS and siRNA lipoplex might be a novel systemic method of delivering siRNA to liver metastasis.
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| Free Research Field |
薬剤学
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