2016 Fiscal Year Final Research Report
Accumulation of proteins utilizing the ring-structure formed by alpha6 and alpha7 subunits of human 20S proteasome
| Project/Area Number |
26460051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Meijo University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | タンパク質 / 集積化 / プロテアソーム / リング |
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| Outline of Final Research Achievements |
The aim of this study is accumulation of proteins based on the homo-double ring formed by alpha7 subunits of human 20S proteasome. GFP, employed as model protein, was fused at N- or C-terminus of alpha7. Resultant proteins did not show ring-formation. Therefore, hetero-ring formation using alpha6 subunit fused with GFP at the N- or C-terminus was attempted. GFP-alpha6 formed hetero-ring efficiently with monomeric form of alpha7, but did not with alpha7 homo-double ring. On the other hand, alpha6-GFP easily formed hetero-ring both with monomeric or oligomeric alpha7. Moreover, GFP-alpha6-GFP formed hetero-ring with monomeric alpha7, indicating that such fusion is effective for higher accumulation of proteins on the ring.
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| Free Research Field |
生物物理
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