ABC protein subfamily D: Mechanisms about subcellular localization and substrate transport, and regulation of cell function

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: University of Toyama
• Principal Investigator: Imanaka Tsuneo 富山大学, 大学院医学薬学研究部(薬学), 教授 (50119559)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ABCトランスポーター / ペルオキシソーム / リソソーム / オルガネラ局在化機構 / 獄長鎖脂肪酸CoA輸送 / ビタミンB12輸送 / 副腎白質ジストロフィー / ビタミンB12欠乏症

Outline of Final Research Achievements

We analyzed mechanism of substrate transport by ABCD1-3 on peroxisomal membrane and mechanism of translocation of ABCD4 to lysosome.
It is revealed that ABCD1 possesses acyl-CoA thioesterase activity and its activity is essential for the transport of acyl-CoA into peroxisome. In addition, it is revealed that ABCD4 makes complex with lysosomal protein LMBD1 on endoplasmic reticulum membranes and translocates from ER to lysosome dependent on lysosomal targeting signal of LMBD1. Furthermore, we succeeded to prepare proteoliposome of ABCD1-4. It becomes possible to characterized precise mechanism of substrate transport by ABCD1-4.

Free Research Field

生化学・分子細胞生物学