KSHV K-RTA-mediated regulation of immunosuppressive cytokine

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Keio University
• Principal Investigator: NOGUHI Kohji 慶應義塾大学, 薬学部(芝共立), 准教授 (80291136)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ウイルス / 転写因子 / サイトカイン / インターロイキン / KSHV

Outline of Final Research Achievements

In this study, we have investigated molecular mechanism involved in KSHV K-RTA-mediated regulation of IL-10. At the specific region on IL-10 gene promoter 256 base upstream from first ATG codon, we identified K-RTA-responsive region, and in addition, we found that some host cell factor(s) seemed to modulate K-RTA-mediated IL-10 promoter activation. We found when K-RTA was co-expressed with some transcriptional factor, synergistic activation of IL-10 promoter was induced, and interestingly, posttranslational modification including protein acetylation had some impact on K-RTA. For protein acetylation modification, there are many members of histone acetylase and deacetylase families known to regulate various transcriptional mechanism so that we would like to determine which molecules were involved in K-RTA-mediated IL-10 promoter activation.

Free Research Field

生物系薬学