2016 Fiscal Year Final Research Report
Generation and characterization of brain- specific UBIAD1-deficient mice using a nestin
| Project/Area Number |
26460085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ビタミンK / UBIAD1 / メナキノン-4 / Nestin-cre / 脳神経 / コンディショナルノックアウトマウス |
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| Outline of Final Research Achievements |
In this study, we created Nestin-cre Ubiad1-CKO mice which were Ubiad1 deficient from the time of neural stem cell. Nestin-cre Ubiad1-CKO was born normally, but these mice were slightly smaller than that of the wild type mice. We measured the Ubiad1 expression and MK-4 concentration in brain tissue at 10 weeks of age. Ubiad1 expression was remarkably low or below the detection limit in any brain tissues. MK-4 concentration was also remarkably low or below the detection limit. We evaluated the brain functions by behavior analysis for 9 to 10 week old Nestin-cre Ubiad1-CKO mice. As a result, short-term memory, anxiety and motor coordination was decreased in Nestin-cre Ubiad1-CKO mice. Moreover, the brain tissue of Nestin-cre Ubiad1-CKO mouse revealed cerebellar atrophy and disappearance of cerebellar Purkinje cells. Therefore, it was suggested that UBIAD1 and MK-4 play an essential role in maintaining structural and function in the cerebellar.
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| Free Research Field |
分子栄養学
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