Melanogenesis regulation by expression and localization of copper-transporters.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Nagasaki International University
• Principal Investigator: Fujita Hideaki 長崎国際大学, 薬学部, 教授 (80291524)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: チロシナーゼ / 銅輸送体 / 銅シャペロン / メラノソーム / リソソーム / ユビキチン / ユビキチンリガーゼ / タンパク質分解

Outline of Final Research Achievements

We have examined a possibility that an activity and expression of tyrosinase, a copper binding proteina and key enzyme of melanin synthesis, is regulated by an intracellular level of copper ion. We found that RNF152, an ubiquitin ligase, specifically interacts with and ubiquitinates tyrosinase. Since RNF152 dose not interacts with and ubiquitinates other melanin synthesis enzyme, Tyrp-1, we concluded that RNF152 may be involved in the regulation of melanin synthesis by controlling an expression level and intracellular localization of tyrosinase in melanocytes. We are unable to show the data that copper-transporters regulate an expression level and intracellular localization of tyrosinase in melanocytes.

Free Research Field

細胞生物学