2017 Fiscal Year Final Research Report
Functional analysis and its application of multifunctional cytochrome P450 enzymes forming carboxyl group
Project/Area Number |
26460132
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Natural medicines
|
Research Institution | Toho University |
Principal Investigator |
ANZAI Yojiro 東邦大学, 薬学部, 教授 (20318299)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Keywords | 抗生物質 / P450酵素 / 酸化反応 |
Outline of Final Research Achievements |
The multifunctional cytochrome P450 enzyme RosC catalyzes a two-step, hydroxylation and alcohol oxidation, oxidation reaction to form the C-20 formyl group in the biosynthesis of a 16-membered macrolide antibiotic rosamicin produced by Micromonospora rosaria IFO13697. In this study, we confirmed that RosC has another oxidation activity, which a formyl group at C-20 of rosamicin is converted into a carboxyl group. Moreover, random mutation analysis was also performed for rosC gene using error-prone PCR, and Leucine-176 of RosC is one of amino acid residue controlling of oxidation reaction.
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Free Research Field |
微生物化学
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