2016 Fiscal Year Final Research Report
Epigenetic manipulation of gene expression in cultured mycobionts for exploring novel bioactive compounds
| Project/Area Number |
26460137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TAKENAKA Yukiko 神戸薬科大学, 薬学部, 准教授 (90289041)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 地衣菌単離培養 / 二次代謝物 / 構造決定 / 生合成 / 生物活性 / エピジェネティック |
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| Outline of Final Research Achievements |
From the cultures of spore-derived mycobionts of the lichens collected in Vietnam and Japan, twenty-nine new metabolites were isolated. Their structures were elucidated by spectroscopic means. Some isolated
compounds exhibited inhibitory activities against mammalian DNA polymerases and cytotoxic effects against human cancer cell lines. Biosynthetic studies and the ITS sequence of the rDNA of cultured mycobionts indicated close phylogenic relationship between a lichen species and some fungi. The epigenetic manipulation of gene expression by HDAC inhibitors failed to influence secondary metabolism in the lichen mycobionts.
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| Free Research Field |
天然物化学
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